ABSTRACT
Cyanobacteria-mediated silver nanoparticles synthesis approach has proven to be more efficient and eco-friendlyin achieving biomedical applications compared to physical and chemical prototypes. In the present work, the silvernanoparticles were successfully synthesized by cell-free extract of freshwater cyanobacteria, i.e., Chroococcus turgidusand Characium typicum. The cyanobacterial silver nanoparticles (CSNPs) were characterized by UV-Vis spectroscopy,scanning electron microscopy (SEM), transmission electron microscopy (TEM), and Fourier transform infrared (FTIR)analysis and were further tested for antibacterial and cytotoxic efficiency. The synthesis of CSNPs was confirmed throughvisible color change and shift of peaks at 430–445 nm by the UV-Vis spectroscopy. The size of CSNPs was between22 and 34 nm and oval-shaped which were confirmed by SEM and TEM analyses. The FTIR spectra showed a newpeak at the range of 3,400–3,460 cm−1 compared to control, confirming the reduction of silver nitrate. Furthermore, theantibacterial activity of CSNPs showed highest zone of inhibition with 6.9, 4.0, 2.0, and 3.0 mm against Salmonellaparatyphi, Escherichia coli, Klebsiella pneumonia, and Staphylococus aureus, respectively, whereas in vitro cytotoxicactivity of C. typicum and C. turgidus silver nanoparticles showed remarkable IC50 values with 43.3 and 40.9 ug/mlagainst MCF-7 breast cancer cell line and 20.8 and 55.7 ug/ml against HepG2 cancer cell, simultaneously